Our group focuses on developing technology and applications on the combination of optical and optoacoustic microscopy in order to deliver contrast and imaging abilities not available in optical microscopes today. While optical fluorescence microscopy is able to achieve sub-microns resolution even below the diffraction limit in living biological specimens, it is restricted by light scattering to superficial investigations at shallow depths. Optoacoustic microscopy offers absorption contrast, which complements fluorescence signals. Importantly it can be implemented to resolve the origin of ultrasound waves generated in response to short nanosecond-range light pulses propagating through tissue, essentially ‘listening’ to the molecules absorbing light. Acoustic signals scatters less than light in tissues and can enable high resolution imaging at larger depths. Our goal is to merge the advantages of optical and acoustical methods, achieving high resolution imaging at greater depths.
Markus Seeger, Angelos Karlas, Dominik Soliman, Jaroslav Pelisek, Vasilis Ntziachristos, Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma. Photoacoustics 4(3), 102–111 (2016).
D. Soliman, G. J. Tserevelakis, M. Omar, V. Ntziachristos, Combining microscopy with mesoscopy using optical and optoacoustic label-free modes. Scientific Reports 5, 12902 (2015).
George J. Tserevelakis, Dominik Soliman, et al., Hybrid multiphoton and optoacoustic microscope. Optics letters 39.7 (2014): 1819-1822.